Description
Kit for IP/ Co-IP of V5-taggedproteins & sample preparation for mass spectrometry (MS)
Specificity V5-tag sequence motif GKPIPNPLLGLDST.
Applications Identification of V5-tagged proteins & interacting partners by MS Interactome studies Superior processing of IP samples into clean peptide mixtures Intuitive protocol for reproducible results
Details The iST V5-Trap Kit enables researchers to process V5-taggedproteins and their interacting partners for Mass Spectrometry analysis by including the ChromoTek V5-Trap for immunoprecipitation/Co-IP of V5-taggedproteins and the PreOmics iST buffers and cartridges required for bottom-up proteomic sample preparation. This robust method yields purified peptides while dramatically reducing contamination and sample loss. Each kit accommodates up to eight samples and includes pull-down reagent for controls.