Description
Kit for immunoprecipitation (IP) and Co-immunoprecipitation (Co-IP) of RFP- and mCherry fusion proteins & sample preparation for mass spectrometry (MS)
Specificity mCherry, mRFP, mRFPruby, mPlum, tagRFP, mKate2 and many more RFP derivatives, see: Fluorescent protein specificity table
Applications Identification of RFP-fusion proteins & complexesby MS Interactome studies Intuitive protocoland reproducible results Superior processing of IP samples into clean peptide mixtures
Details The iST RFP-Trap Kit forprocessing RFP-fusion proteins and their interacting partners for mass spectrometry analysis: The kit includesthe RFP-Trap for IP/Co-IP of RFP-fusion proteins and the PreOmics iST buffers and cartridges required for bottom-up proteomic sample preparation. This robust method yields purified peptides while dramatically reducing contamination and sample loss. Each kit accommodates up to eight samples and includes pull-down reagent for controls.