ThethermoMACSSeparatorisaheatabledevicedesignedformoleculeisolationandsubsequentin-columnprocessing.ThethermoMACSSeparatorisusedincombinationwithµColumnsandµMACSMicroBeads.TheseparatorcanholduptofourµColumns.Enzymaticreactionswithseparatedmoleculescanbeperformedinthecolumn,asthethermoMACSSeparatorcanbeheatedto37°Cor42°C.
TargetmoleculesmagneticallylabeledwithMACSMicroBeadsareretainedintheµColumnplacedinthethermoMACSSeparator.Thereby,nucleicacids,suchasmRNAaswellasproteins,ormolecularcomplexesareefficientlypurifiedfromcelllysates.SubsequentenzymatictreatmentofmoleculesretainedintheµColumncanbeperformedat37°Cor42°C.Thisenablesin-column
CDNAsynthesisimmediatelyaftermRNAisolationfromcells,enzymaticproteinassays,orDNAmodifications.Suchin-columnreactionssavetimeandtargetmolecules,astube-to-tubetransfer,precipitation,orseparatepurificationstepsaredispensable.