The TFact™, JunD (Phospho-Ser255) DNA-Binding ELISA detects active JunD (Phospho-Ser255) in Jurkat Nuclear Extract. The Jurkat cells were grown 3 days in RPMI 1640 with 10% FBS and harvested for nuclear extract. The Jurkat cells were stimulated by PMA (200nM) before harvest. The DNA-transcription factor complex is treated with the stablization buffer.
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NCBI Gene Symbol
JUND
Specificity
The TFact™, JunD (Phospho-Ser255) DNA-Binding ELISA Kit detects endogenous levels of JunD only when phosphorylated at Ser255.