The TFact™, JunB (Phospho-Ser259) DNA-Binding ELISA detects active JunB (Phospho-Ser259) in Jurkat Nuclear Extract. The Jurkat cells were grown 3 days in RPMI 1640 with 10% FBS and harvested for nuclear extract. The Jurkat cells were stimulated respectively by PMA (200nM) and UV (100J/M2) before harvest. The DNA-transcription factor complex is treated with the stabilization buffer in the Jurkat nuclear extract with PMA stimulation.
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NCBI Gene Symbol
JUNB
Specificity
The TFact™, JunB (Phospho-Ser259) DNA-Binding ELISA Kit detects endogenous levels of JunB only when phosphorylated at Ser259.