当前位置 ： NEB >>> NEB/Monarch® RNA Cleanup Kit (500 µg)/10 preps/T2050S
NEB/Monarch® RNA Cleanup Kit (500 µg)/10 preps/T2050S
  • NEB/Monarch® RNA Cleanup Kit (500 µg)/10 preps/T2050S

NEB/Monarch® RNA Cleanup Kit (500 µg)/10 preps/T2050S

价格: 试用 市场价: 0.00
打印产品信息
货号: T2050S
品牌: NEB
规格
数量
库存(0)
特别 提示
代购产品：无质量问题不接受退换货，下单前请仔细核对信息。
下单后请及时联系客服核对商品价格，订单生效后再付款。
立即申请

收藏商品
资深产品顾问
咨询顾问

全国免费服务热线

4000-520-616

  • 自营商城 一站式服务
  • 厂家直采 剔除溢价
  • 品质甄选 正品保证
  • 严控流程 只做188精品
  • 极速物流 如约送货
  • 详情
  • 使用说明
  • 常见问题

    • The Monarch RNA Cleanup Kit (500 µg) reliably purifies up to 500 μg of concentrated, high-quality RNA (> 25 nt) from enzymatic reactions and in vitro transcription (IVT) reactions. The kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. The columns ensure zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 50 μl. Unwanted NTPs and short RNA fragments are removed, ensuring highly pure RNA transcripts following IVT/RNA synthesis. Eluted RNA is ready for use in a variety of downstream applications including RT-PCR, RNA Library Prep for NGS and RNA labelling. The protocol can also be modified to enable the purification of smaller RNA fragments (≥ 15 nt).

      Designed with sustainability in mind, Monarch kits use significantly less plastic and responsibly-sourced, recyclable packaging.

      Monarch RNA Cleanup Kits are also available for 10 µg (NEB #T2030) and 50 µg (NEB #T2040) binding capacities. Columns and buffers are also available separately for convenience.

      Figure 1: Monarch RNA Cleanup Kit workflow

      Specifications and Applications:

      SPECIFICATIONS
      RNA Sample TypeCleanup of RNA from large-scale in vitro transcription reactions
      Binding Capacity500 µg
      RNA Size Range≥ 25 nt ( ≥ 15 nt with modified protocol)
      Typical Recovery70–100%
      Elution Volume50–100 µl
      PurityA260/280 > 1.8 and A260/230 > 1.8
      Protocol Time10–15 minutes of spin and incubation time
      Compatible Downstream ApplicationsRNA Labeling, RNAi, Microinjections, RT-PCR, RNA library prep for NGS, transfection
      APPLICATIONS
      RNA Cleanup and Concentration (including from the TRIzol aqueous phase)RNA purified by other methods can be further purified
      Enzymatic Reaction CleanupEnzymes such as RNA polymerases, DNase I, Proteinase K and phosphatases are removed allowing efficient desalting
      In vitro Transcription CleanupEnzymes and excess NTPs are removed to yield highly pure synthesized RNA
      RNA Gel ExtractionPurification of RNA from agarose gels
      RNA FractionationFractionation of RNA into small and large RNA pools

      Figure 2: The Monarch RNA Cleanup Kit (500 µg) is suitable for cleaning up large quantities (>250 µg) of RNA from in vitro transcription reactions

      A. RNA transcripts of varying sizes (0.6-8 kb) were synthesized using the HiScribe®; T7 Quick High Yield RNA Synthesis Kit (NEB #E2050) using 1.5-1.8 µg of DNA template for two hours at 37°C. 40 µl of each in vitro transcription IVT) reaction was cleaned up using the Monarch RNA Cleanup Kit (500 µg, T2050) and eluted in 200 µl. RNA yields were calculated from the resulting A260, measured using a Nanodrop® spectrophotometer and ranged from 268-425 µg of RNA per IVT reaction.B. RNA integrity (200 ng/lane) was assessed on a 1% agarose-TBE gel stained with SYBR® Gold.
      Figure 3: The Monarch RNA Cleanup Kit (500 µg) cleans up large-scale in vitro transcription reactions and generates yields consistent with other large-scale cleanup kits

      0.3 and 1.8 kb fragments were in vitro transcribed at 37°C (overnight and 2 hours, respectively) using the HiScribe®; T7 Quick High Yield RNA Synthesis Kit (NEB #E2050). Following DNase I treatment (4 U DNase I, 37°C, 15 minutes), transcription reactions were pooled and 200 µl cleaned up using either the NEB Monarch RNA Cleanup Kit (500 µg) or the MEGAclear Transcription Clean-Up Kit (Thermo Fisher Scientific). In vitro transcribed RNA was eluted twice with 100 µl of nuclease-free water following a 5-minute on-column incubation (room temperature for Monarch and 65°C for MEGAclear. Recovery of the synthesized RNA transcript was calculated from the resulting A260 using a Trinean Dropsense™ 16. The Monarch RNA Cleanup Kit (500 µg) produces similar RNA yields as the MEGAclear Kit for large-scale in vitro transcription reactions.
      Figure 4: RNA recovery is increased by incubating the column with the elution buffer (nuclease-free water) prior to the elution spin

      rRNA (16S and 23S Ribosomal Standard from E.coli, Sigma) was purified using the Monarch RNA Cleanup Kit (500 µg, NEB #T2050). 100 µl of nuclease-free water was added to the column and incubated for either 0,1,3 or 5 minutes at room temperature before spinning to elute the RNA. The percent recovery of RNA was calculated from the resulting A260 as measured using a Trinean Dropsense™ 16. A five-minute incubation period produced the maximum yield.
      This product is related to the following categories:
      RNA Cleanup Products,
      RNA Reagents Products,
      RNA Extraction & Purification Products,
      Nucleic Acid Purification Products
      This product can be used in the following applications:
      RNA Purification and Isolation,
      RNA Analysis
    售后保障
    蚂蚁淘生物188，秉承蚂蚁淘一贯的严谨态度，由蚂蚁淘公司专业人员负责品控、采购、物流、销售、售后，保障正品优质。以“快速好省，为科研提供好产品、好价格”为理念，直接链接原厂家，从全国各地原制造商严格挑选188款科研精品，剔除品牌溢价，188生物新电商，把好的产品带给科研！  力求给你最优质的商品。
  • Q：生物188产品正品保障吗？
    A：生物188质量把控人员具有十年的从业经验，在业界享有良好的口碑；自营商城，直接从厂家采购， 自己的团队负责国际物流和清关，中间没有第三方，所有流程严格把控，100%保证正品，假一罚十。

    Q：下单后可以修改订单吗？
    A：下单后的商品付款之前可以修改；订单付款成功，需要联系我们客服进行修改；客服电话：4000-520-616

    Q：可以开发票吗？
    A：本网站所售商品都是正规清关，均开具16%正规发票，发票金额含配送费金额，另有说明的除外。

    Q：商品几天可以发货？
    A：生物188商品，全部现货销售，付款后即可发货,一般一周内送达！

    Q：如何联系商家？
    A：有任何问题够可以电话咨询我们，全国24小时免费服务热线：4000-520-616 或联系我们的在线客服QQ：1570468124

    Q：收到的商品少了/发错了怎么办？
    A：同个订单购买多个商品可能会分为一个以上包裹发出，可能不会同时送达，建议查看订单详情是否是 部分发货状态；如未收到，可联系在线客服或者致电4000-520-616。

    Q：退换货/维修需要多长时间？
    A：一般情况下，退货处理周期为客户收到产品一个月内（以快递公司显示签收时间为准），包装规格、 数量、品种不符，外观毁损、短缺或缺陷，请在收到货24小时内申请退换货；特殊商品以合同条款为准。

客户服务

在线客服
用户反馈

何为188

极简而严谨，我们仅销售188款生物医学科研用品，款款都是爆款；因为少所以聚焦，聚焦甄选每一款产品，聚焦服务每一位客户!

关注我们 :

点击QQ联系我们
生物188微信

关注188微信公众号

获取最新优惠活动通知

  • 品质甄选，正品保证

  • 自营电商，厂家直采

  • 极简主义，188精品

常见问题 | 购买相关 | 配送与验收 | 支付问题 | 售后与服务 | 服务协议 | 隐私政策 | 何为188 | 中国智造 | 联系我们
友情链接： HansaBioMed产品 | Polysciences经销商 | Amaxa公司 | Biomatik pH标准试剂盒 | Sage Science华东代理 | Life Sensors代理 | advancedbiochemicals | 蚂蚁淘 | 北京鑫盛鸿阳科技有限公司

公司版权所有 © 2005-2021 苏州蚂蚁淘生物科技有限公司 苏ICP备17049038号-10