The Monarch RNA Cleanup Columns (50 µg) are a component of the Monarch RNA Cleanup Kit (50 µg) (NEB #T2040) and can be used to purify up to 50 µg of RNA from enzymatic reactions including DNase treatment (NEB #M0303), labeling, and capping. The columns are ideal for cleaning up RNA after in vitro transcription reactions such as sgRNA synthesis reactions (NEB #E3322). The columns are designed without the use of a retaining ring, ensuring no buffer retention and no carryover of contaminants. RNA can be eluted in as little as 20 μl for and is ready for use in a variety of downstream applications including RT-PCR, RNA Library Prep for NGS and transfection.
Monarch RNA Cleanup Columns are also available for 10 µg (NEB #T2037) and 500 µg (NEB #T2057) binding capacities.
Figure 1: The Monarch RNA Cleanup Kit enables efficient recovery of RNA in as little as 20 µl
rRNA (50 µg of 16S and 23S Ribosomal Standard from E. coli, Sigma) was purified using the Monarch RNA Cleanup Kit (50 µg, NEB #2040) and eluted with nuclease-free water using the elution volumes indicated. The percent recovery of RNA was calculated from the resulting A260, as measured using a Trinean DropSense 16. Approximately 80% of RNA can be efficiently recovered in as little as 6 µl.Figure 2: The Monarch RNA Cleanup Kit (50 µg) produces sgRNA yields consistent with other competitor RNA cleanup kits and with lower residual NTP contamination
Six different sgRNA synthesis reactions from the EnGen® sgRNA Synthesis Kit, S. pyogenes (NEB #E3322) were cleaned up using either the Monarch RNA Cleanup Kit (50 µg, NEB #T2040) or a competitor kit (according to manufacturer’s recommendations) and eluted with 50 µl of nuclease-free water. sgRNA yield was calculated from the resulting A260, measured using a Trinean Dropsense 16. The Monarch Cleanup Kit produced sgRNA yields consistent with other commercially available RNA cleanup kits.
This product is related to the following categories:
RNA Cleanup Products,
RNA Reagents Products,
RNA Extraction & Purification Products,
Nucleic Acid Purification Products
This product can be used in the following applications:
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