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NEB/Monarch® RNA Cleanup Kit (10 µg)/10 preps/T2030S
  • NEB/Monarch® RNA Cleanup Kit (10 µg)/10 preps/T2030S

NEB/Monarch® RNA Cleanup Kit (10 µg)/10 preps/T2030S

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货号: T2030S
品牌: NEB
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    • The Monarch RNA Cleanup Kit (10 µg) rapidly and reliably purifies up to 10 μg of concentrated, high-quality RNA (> 25 nt) from enzymatic reactions including labeling, capping, in vitro transcription (IVT) and DNase I treatment. This kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. Our unique column design ensures zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 6 μl. Eluted RNA is ready for use in a variety of downstream applications, including RT-PCR, RNA Library Prep for NGS and RNA labelling. The protocol can also be modified to enable the purification of smaller RNA fragments (≥ 15 nts).

      Designed with sustainability in mind, Monarch kits use significantly less plastic and responsibly-sourced, recyclable packaging.

      Monarch RNA Cleanup kits are also available for 50 µg (NEB #T2040) and 500 µg (NEB #T2050) binding capacities. Columns and buffers are also available separately for convenience.

      Figure 1: Monarch RNA Cleanup Kit workflow

      Specifications andApplications:

      SPECIFICATIONS
      RNA Sample TypeCleanup and concentration of RNA from enzymatic reactions (labeling, capping, in vitrotranscription reactions, DNase I treatment)
      Binding Capacity10 μg
      RNA Size Range≥ 25 nt ( ≥ 15 nt with modified protocol)
      Typical Recovery70–100%
      Elution Volume6–20 µl
      PurityA260/280 > 1.8 and A260/230 > 1.8
      Protocol Time5 minutes of spin and incubation time
      Compatible Downstream ApplicationsRT-PCR, Small RNAlibrary prep for NGS, RNA Library Prep for NGS
      APPLICATIONS
      RNA Cleanup and Concentration (including from the TRIzol aqueous phase)RNA purified by other methods can be further purified
      Enzymatic Reaction CleanupEnzymes such as RNA polymerases, DNase I, Proteinase K and phosphatases are removed allowing efficient desalting
      In vitro Transcription CleanupEnzymes and excess NTPs are removed to yield highly pure synthesized RNA
      RNA Gel ExtractionPurification of RNA from agarose gels
      RNA FractionationFractionation of RNA into small and large RNA pools

      Figure 2: The Monarch RNA Cleanup Kit enables efficient recovery of RNA in as little as 6 µl

      rRNA (10 µg of 16S and 23S Ribosomal Standard from E. coli, Sigma) was purified using the Monarch RNA Cleanup Kit (10 µg, NEB #2030) and eluted with nuclease-free water using the elution volumes indicated. The percent recovery of RNA was calculated from the resulting A260 as measured using a Trinean DropSense 16. ~80% of RNA can be efficiently recovered in as little as 6 µl.
      Figure 3: The Monarch RNA Cleanup Kit enables efficient recovery of <20 ng of RNA in as little as 6 µl

      A 2-fold dilution series (from 1000 to 15.6 ng) of rRNA (16S and 23S Ribosomal Standard from E.coli, Sigma) was purified using the Monarch RNA Cleanup Kit (10 µg, NEB #1030) and eluted in 6 µl of nuclease-free water. The percent recovery of RNA was calculated from the resulting A260, measured using a Trinean DropSense 16. Even low RNA inputs (<20 ng) are efficiently cleaned up and recovered (>80%) in as little as 6 µl.
      Figure 4: The Monarch RNA Cleanup Kit (10 µg) offers efficient recovery of sample volume

      The Monarch RNA Cleanup Columns (10 µg, NEB #T2037) ensure zero buffer retention and no carryover of contaminants, enabling sample elution in volumes as low as 6 µl. Moreover, ~90% (~5.4 µl) of the minimal elution volume is recovered from the Monarch RNA Cleanup Columns, compared to other competitor kits.
      This product is related to the following categories:
      RNA Cleanup Products,
      RNA Reagents Products,
      RNA Extraction & Purification Products,
      Nucleic Acid Purification Products
      This product can be used in the following applications:
      RNA Purification and Isolation,
      RNA Analysis
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