The Monarch DNA Gel Extraction Kit rapidly and reliably purifies up to 5 μg of concentrated, high-quality DNA from agarose gels. The kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. The columns ensure zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 6 μl. The buffers provided have been optimized, and do not require monitoring of pH. Unlike other kits, there is no need to add isopropanol to the melted agarose prior to loading on the column, saving you a step. Eluted DNA is ready for use in restriction digests, DNA sequencing, ligation and other enzymatic manipulations.Designed with sustainability in mind, Monarch kits use significantly less plastic and responsibly-sourced, recyclable packaging
View our videos on protocols, tips, and recycling Monarch.
Monarch DNA Cleanup Column DesignMonarch columns are designed for performanceMonarch columns are designed without a frit, which eliminates buffer retention and the risk of carryover contamination, providing fast, worry-free DNA purification.Advantages:
Elute in as little as 6 μl
Prevent buffer retention and salt carry-over with optimized column design
Save time with fast, user-friendly protocols
No need to monitor pH or add isopropanol
Buffers and columns available separately
Significantly less plastic used when compared with other kits
Responsibly-sourced and recyclable packaging
Specifications
DNA Sample Type:
double-stranded DNA from agarose gels
Binding Capacity:
up to 5 μg
DNA Size Range:
~50 bp to 25 kb
Typical Recovery:
DNA (50 bp to 10 kb): 70–90%DNA (11–23 kb): 50–70%
Elution Volume:
≥ 6 μl
Purity:
A260/280 > 1.8
Protocol Time:
10 minutes of spin and incubation time
Compatible DownstreamApplications:
ligation, restriction digestion, labeling and other enzymaticmanipulations, library construction and DNA sequencing.
Monarch DNA Gel Extraction Kit ProtocolDNA purified from the Monarch DNA Gel Extraction Kit is recovered with similar efficiency and purity as the leading supplier, but is more highly concentrated, facilitating its use in downstream applicationsOne microgram aliquots of a 3 kb fragment were resolved on a 1% w/v agarose gel, excised, and processed with different kits using manufacturer-specified minimum elution volumes. Values reported are the concentration and purity data determined by Nanodrop™ readings, as well as recovery calculations based on the eluted DNA concentration and recovered volume.Monarch DNA Gel Extraction Kit reproducibly recovers DNA over a broad range of molecular weights.A mixture of 7 DNA fragments ranging from 10 kb down to 0.5 kb was prepared and one-half of the mixture was resolved on a 1% gel. Each fragment was manually excised from the agarose gel and processed using the Monarch DNA Gel Extraction Kit. The entire elution of each fragment was resolved on a new gel with the remainder of the original mixture for comparison.DNA purified from agarose gels using the Monarch DNA Gel Extraction Kit can be reproducibly isolated and ligated.Two micrograms of a 3 kb fragment with compatible ends was resolved on a 1% agarose gel, excised, and purified using the Monarch DNA Gel Extraction Kit. Samples were eluted in 20 μl and a fraction (1/4 th of total) was ligated using the Blunt/TA Ligase Master Mix (NEB #M0367). Representative samples from 5 replicates were resolved on a second 1% agarose gel. M is the 1 kb DNA Ladder (NEB #N3232).
This product is related to the following categories:
DNA Gel Extraction Products,
Nucleic Acid Purification Products
This product can be used in the following applications:
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