| Description: | The intestines are a long and continuous tube that run from stomach to anus. Mesentery is a fold of tissue that attaches intestines to the body wall. Structurally, intestines and mesentery are very different. However, from a protein extraction point of view, they have one thing in common: they are very tough and hard to homogenize. It is also very difficult to lyse cells in the tissues for total protein extraction. Traditional solution-based protein extraction methods, such as RIPA, are inefficient and the protein yield is very low. The profile of extracted protein using traditional methods is usually incomplete. This kit provides a highly efficient method for total protein extraction from human or animal intestines and mesentery tissues using a combination of mechanical extraction and chemical lysis. The cell lysis buffers used are much stronger than RIPA buffers. The kit features a simple, fast single-tube protocol and optimized buffers for intestine and mesentery tissues. Researchers have the option to choose either a denaturing or native cell lysis buffer for specific downstream applications. The whole procedure takes less than 10 minutes to complete and the protein yield is in the range of 1-3 mg/ml. The materials provided are sufficient for 50 extractions. |
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