| Description: | Skin tissue is made up of epidermis, dermis and subcutaneous fat. Because of its unique structure, skin tissue is notoriously difficult to homogenize. It is also very difficult to lyse the cells in the tissue for total protein extraction. Traditional solution-based protein extraction methods, such as RIPA, are inefficient and the yield is very low. The profile of extracted protein is also incomplete with solution-based methods. This kit provides a highly efficient method for total protein extraction from human or animal skin tissues by using a combination of mechanical extraction and chemical lysis of skin tissues. The kit features a simple and fast single-tube protocol and optimized buffers for skin tissues. The researchers have the option to choose either a denaturing or native cell lysis buffer, which is specifically tailored for skin tissue. The whole procedure takes less than 10 minutes to complete and the protein yield is in the range of 1-5 mg/ml. The materials provided are sufficient for 50 extractions. |
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