This package includes 50 nmol of CLIP-Surface 547 substrate, sufficient to make 10 ml of a 5 µM CLIP-tag fusion protein labeling solution.The CLIP-tag protein labeling system enables the specific, covalent attachment of virtually any molecule to a protein of interest. CLIP-tag is a protein tag based on human O6-alkylguanine-DNA-alkyltransferase (hAGT). CLIP-tag substrates are derivatives of benzylcytosine (BC). In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the reactive cysteine of CLIP-tag forming a stable thioether bond. Although CLIP-tag is based on the same protein as SNAP-tag®, the benzylcytosine substrates form a separate class of substrates, different from the benzylcytosine substrates form a separate class of substrates, different from the benzylguanine substrates recognized by SNAP-tag. CLIP-tag and SNAP-tag can be used for orthogonal and complementary labeling of two proteins simultaneously in the same cells.There are two steps to using this system: subcloning and expression of the protein of interest as a CLIP-tag fusion, and labeling of the fusion with the CLIP-tag substrate of choice. Expression of CLIP-tag fusion proteins is described in the documentation supplied with CLIP-tag plasmids. The labeling of the fusion proteins on the cell surface with the CLIP-tag substrate is described in this document.Figure 1:Live CHO-K1 cells transiently transfected with pCLIP-NK1RCells were labeled with CLIP-Surface 547 (red) for 60 minutes and counterstained with Hoechst 33342 (blue).Figure 2:Excitation (dotted line) and emission (full line) spectra of CLIP-Surface 547 coupled to CLIP-tag in buffer at pH 7.5Figure 3. Structure of CLIP-Surface 547 (MW 851.0 g/mol)
This product is related to the following categories:
CLIP-tag™ Substrates Products,
Protein Labeling Products
This product can be used in the following applications:
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