Two ml of SNAP-Capture Magnetic Beads is sufficient to perform 25 immobilization assays using 80 µl of bead suspension per assay. The SNAP-tag is a novel tool for protein research, allowing the specific, covalent attachment of virtually any molecule or modified surface to a protein of interest. The SNAP-tag is a protein based on mammalian O6-alkylguanine-DNA-alkyltransferase (AGT). SNAP-tag substrates are derivatives of benzyl purines and benzyl pyrimidines. In the immobilization reaction, the SNAP-tag is covalently attached to the substituted benzyl group of the SNAP-Capture Magnetic Beads.
There are two steps to using this system: sub-cloning and expression of the protein of interest as a SNAP-tag fusion, and capture and immobilization of the fusion protein using SNAP-Capture Magnetic Beads.
For larger volume requirements, customized and bulk packaging is available by purchasing through the OEM/Bulks department at NEB. Please contactcustom@neb.comfor further information.
This product is related to the following categories:
Snap Capture Products,
Proteome Analysis Products,
Protein Labeling Products
This product can be used in the following applications: