This package includes 50 nmol of SNAP-Surface Alexa Fluor 488 substrate, sufficient to make 10 ml of a 5 µM SNAP-tag fusion protein labeling solution.The SNAP-tag is a novel tool for protein research,allowing the specific, covalent attachment ofvirtually any molecule to a protein of interest.The SNAP-tag is based on mammalian O6-alkylguanine-DNA-alkyltransferase (AGT). SNAP-tagsubstrates are derivatives of benzylguanines andbenzylchloropyrimidines. In the labeling reaction,the substituted benzyl group of the substrate iscovalently attached to the SNAP-tag.There are two steps to using this system: subcloning and expression of the protein of interest as a SNAP-tag fusion, and labeling of the fusion with the SNAP-tag substrate of choice. Expression of SNAP-tag fusion proteins is described in the documentation supplied with SNAP-tag plasmids. The labeling of the fusion proteins with the SNAP-tag substrate is described below.Figure 1:Live HEK293 cells transiently transfected with pSNAP-ADRβ2 and pSNAP-H2BCells were sequentially labeled with SNAP-Surface Alexa Fluor 488 (green) and SNAP-Cell TMR-Star (red) for 15-30 minutes.Figure 2:Excitation (dotted line) and emission (full line) spectra of SNAP-Surface Alexa Fluor® 488 coupled to SNAP-tag in buffer at pH 7.5
This product is related to the following categories:
SNAP-tag Substrates Products ,
Protein Labeling Products
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