This package includes 50nmol of SNAP-Surface 488, sufficient to make 10 ml of a 5 µM SNAP-tag fusion protein labeling solution.The SNAP-tag protein labeling system enables the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is a small protein based on mammalian O6-alkylguanine-DNA-alkyltransferase (AGT). SNAP-tag substrates are derivatives of benzylpurines and benzylchloropyrimidines, In the labeling reaction, the substituted benzyl group of the substrate becomes covalently attached to the SNAP-tag.There are two steps to using this system: sub-cloning and expression of the protein of interest as a SNAP-tag fusion, and labeling of the fusion with the SNAP-tag substrate of choice. Expression of SNAP-tag fusion proteins is described in the documentation supplied with SNAP-tag plasmids. The labeling of the fusion proteins with the SNAP-tag substrate is described below.Figure 1:Live HEK293 cells transiently transfected with pSNAP-ADRβ2Cells were labeled with SNAP-Surface 488 (green) for 30 minutes.Figure 2:Structure of SNAP-Surface 488 (MW 841.9 g/mol)Figure 3:Excitation (dotted line) and emission (solid line) spectra of SNAP-Surface 488 coupled to SNAP-tag in buffer at pH 7.5
This product is related to the following categories:
SNAP-tag Substrates Products ,
Protein Labeling Products
This product can be used in the following applications:
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