This package contains 50nmol of SNAP-Cell 505-Starsubstrate, sufficient to make 10 ml of a 5 µM SNAP-tag fusion protein labeling solution.The SNAP-tag protein labeling system enables the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is a protein based on human O6-alkylguanine-DNA alkyltransferase (hAGT). SNAP-tag substrates arefluorophores, biotin or beads conjugated to guanine or chloropyrimidine leaving groups via a benzyl linker. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag.There are two steps to using this system: subcloning and expression of the protein of interest as a SNAP-tag fusion, and labeling of the fusion with the SNAP-tag substrate of choice. Expression of SNAP-tag fusion proteins is described in the documentation supplied with SNAP-tag plasmids. The labeling of the fusion proteins with the SNAP-tag substrate is described below.Figure 1:Live U-2 OS cells stably transfected with pSNAPf-H2BCells were labeled with SNAP-Cell 505-Star(green) for 15 minutes at 37°C, 5% CO2.Figure 2:Excitation (dotted line) and emission spectra of SNAP-Cell 505-Star coupled to SNAP-tag in buffer at pH 7.5Figure 3.Structure of SNAP-Cell 505-Star(MW 621.0 g/mol).
This product is related to the following categories:
SNAP-tag Substrates Products ,
Protein Labeling Products
This product can be used in the following applications:
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