NEBExpress® Ni Resin is an affinity matrix for the isolation and purification of polyhistidine-tagged (His-tagged) fusion proteins. It is intended for use in gravity or pressure flow columns, and batch purifications. NEBExpress Ni Resin is comprised of a highly uniform and stable chemical-tolerant resin, pre-charged with nickel ions on the matrix surface. It is resistant to a wide range of chemicals, including NaOH, EDTA, DTT and β-Mercaptoethanol.For larger volume requirements, customized and bulk packaging is available by purchasing through the OEM/Bulks department at NEB. Please contact custom@neb.com for further information.
Support Matrix: Spherical, agarose based microparticles ranging in size from 10-100 μm.
- This product is related to the following categories:
- Amylose Purification (MBP-tag),
- NEBExpress MBP Fusion and Purification System,
- Nickel Purification (His-tag),
- Bacterial E. coli Protein Expression,
- Affinity Purification,
- Protein Purification,
Protein Expression
- This product can be used in the following applications:
- MBP Affinity Tag,
- Fusion Protein Cleavage,
- His-tagged Protein Expression & Purification,
- Pull Down Assays,
- Affinity Purification & Expression Tags,
- Protein Purification,
Protein Analysis Tools