Spin columns containing an affinity matrix for the small-scale isolation and purification of polyhistidine-tagged (His-tagged) fusion proteins. Immobilized Metal Affinity Chromatography (IMAC) purifications employing NEBExpress® Ni Spin columns can be performed under native or denaturing conditions, yielding highly pure target in a single protein purification step. This enables screening of expression conditions and streamlines the functional and structural characterization of the target protein.
Support Matrix: Spherical, agarose based microparticles ranging in size from 10-100 μm.
This product is related to the following categories:
Amylose Purification (MBP-tag),
Nickel Purification (His-tag),
Affinity Purification,
Protein Purification
This product can be used in the following applications: