Linker/RNA Ligation Protocol: The Universal miRNAcloning linker has a 3´ blocking group (amine) to prevent self ligation,circularization and ligation to RNA at the 5´ end. It also contains the BanIrestriction site compatible with the miRNA cloning protocol used by Bartel(1).For ligation, resuspend the lyophilized oligonucleotide inRNAse-free water to 100 μM stock concentration. (A resuspension volume of8.6 μl will give a 0.58 μg/μl or 100 μM stocksolution).Ligations are carried out with T4 RNALigase I in the absence of ATP. Approximately 1 μg is typically used perreaction. See reference 1 for a detailed protocol.
Sequence
5´ rAppCTGTAGGCACCATCAAT–NH2 3´
This product is related to the following categories:
购物车为空，快去购物吧！
4000-520-616
咨询顾问
特别 提示
