Recombinant human antibody raised against SARS-CoV-2 S ECD.
Immunogen:
Natural SARS-CoV-2.
Host:
Human
Reactivity:
SARS-CoV-2
Specificity:
This antibody detects endogenous levels of S-ECD protein.
Form:
Liquid
Purification:
Protein A purification
Purity:
> 95% (by SDS-PAGE).
Isotype:
IgG
Recommend Usage:
ELISA (1:160000)NeutralizationWestern Blot (1:500-1:1000)The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS, pH 7.2.
Storage Instruction:
Store at 4°C for short term. For long term storage, store at -20°C.Aliquot to avoid repeated freezing and thawing.
Antibody Species:
Human
Source / Expression System:
293F
Matched Pair:
RAB01045 with RAB01048; RAB01046 with RAB01048; RAB01047 with RAB01048
Datasheet:
Download
Applications
Neutralization
Western Blot (Recombinant protein)
Western Blot analysis of Lane 1: S1 (SARS-CoV-2) RBD Recombinant Protein (Cat # P6661) 1 ug and Lane 2: S (SARS-CoV-2) ECD Recombinant Protein (Cat # P6662) 1 ug. Primary antibody: SARS-CoV-2 S ECD recombinant human antibody, clone BHB04 (Cat # RAB01048) (1:500). Secondary antibody: Goat anti-human IgG+HRP (1:5000). Exposure time: 30 s.
Enzyme-linked Immunoabsorbent Assay
An ELISA was performed using 1. Coating original concentration: 2 ug/mL, 100 uL/hole sample is S1 (SARS-CoV-2) RBD Recombinant Protein (Cat # P6661) and S (SARS-CoV-2) ECD Recombinant Protein (Cat # P6662) 2. Primary antibody: SARS-CoV-2 S ECD recombinant human antibody, clone BHB04 (Cat # RAB01048) 3. Secondary antibody: Goat anti-human IgG+HRP (1:10000) 4. Develop: 15 min, 100 uL/hole 5. Stop: stop buffer 50 uL/hole 6. ELISA result are shown in the figure.
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