Recombinant human antibody raised against SARS-CoV-2 S ECD/RBD.
Immunogen:
Natural SARS-CoV-2.
Host:
Human
Reactivity:
SARS-CoV-2
Specificity:
The antibody detects endogenous levels of SARS-CoV-2 S ECD/RBD.
Form:
Liquid
Purification:
Affinity purification
Purity:
> 95% by SDS-PAGE
Isotype:
IgG
Recommend Usage:
ELISANeutralizationWestern Blot (1:1000-1:2000)The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS, pH 7.2.
Storage Instruction:
Store at 4°C for short term. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Antibody Species:
Human
Source / Expression System:
293F
Matched Pair:
RAB01048 (for SARS-CoV-2 S ECD)
Datasheet:
Download
Applications
Western Blot
Western blot analysis of Lane 1: S1 (SARS-CoV-2) RBD Recombinant Protein (Cat # P6661), Lane 2: S (SARS-CoV-2) ECD Recombinant Protein (Cat # P6662) with SARS-CoV-2 S ECD/RBD recombinant human antibody, clone BHB03 (1:4000) and Goat Anti-Human IgG (H&L) secondary antibody (HRP) (1:5000).
Neutralization
Enzyme-linked Immunoabsorbent Assay
ELISA analysis was coated by Lane 1: S1 (SARS-CoV-2) RBD Recombinant Protein (Cat # P6661), Lane 2: S (SARS-CoV-2) ECD Recombinant Protein (Cat # P6662), 100 uL/well at 2 ug/mL, was performed by loading SARS-CoV-2 S ECD/RBD recombinant human antibody, clone BHB03 at various concentrations and Goat Anti-Human IgG (H&L) secondary antibody (HRP) (1:10000).
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