Recombinant human antibody raised against SARS-CoV/SARS-CoV-2 N.
Reactivity:
SARS-CoV, SARS-CoV-2
Specificity:
This antibody recognizes human SARS Coronavirus Nucleocapsid protein.
Form:
Liquid
Purification:
Protein A purification
Purity:
> 98% (SDS-PAGE)
Isotype:
IgG1
Quality Control Testing:
Antibody Reactive Against Recombinant Proteins.Western Blot analysis of different recombinant proteins were stained with this antibody at 1:1000 dilution. Lane 1: SARS-CoV-2 NP, Lane 2: SARS-CoV NP, Lane 3: MERS-CoV NP and Lane 4: HCoV-NL63 NP.
Recommend Usage:
ELISA (1:5000-1:20000)Flow Cytometry (1:500-1:1000)Immunofluorescence(1:500-1:1000)Western Blot (1:1000-1:5000)The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS, pH 7.4.
Storage Instruction:
Store at 4°C for at least 6 months. For long term storage, store at -20°C.Aliquot to avoid repeated freezing and thawing.
Note:
Multi-cycle kinetic analysis
Multi-cycle kinetic analysis of this antibody to human SARS-CoV-2 Nucleocapsid protein by using Biacore T200.
Antibody Species:
Human
Source / Expression System:
293
Datasheet:
Download
Applications
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: HEK293 cells and Lane 2: transfected with SARS-CoV-2 VLP (Virus-like particle) were stained with this antibody at 1:1000 dilution.
Western Blot (Recombinant protein)
Immunofluorescence
Immunofluorescent staining of 293T cells were respectively transfected with SARS-CoV, SARS-CoV-2, MERS-CoV and HCoV-NL63 nucleocapsid plasmid and stained with this antibody (1:500).
Enzyme-linked Immunoabsorbent Assay
ELISA assay of this antibody to SARS-CoV-2 nucleocapsid protein, SARS-CoV nucleocapsid protein, MERS-CoV nucleocapsid protein, HCoV-NL63 nucleocapsid protein and BSA (BSA: negative control).