Recombinant His-tagged mouse Fab fragment raised against c-Myc tag.
Immunogen:
Original antibody is raised against a synthetic peptide corresponding to amino acids 408-439 of human c-myc.
Reactivity:
Human
Specificity:
The epitope sequence, EQKLISEEDL, is located in aa 410-419 of human c-myc protein.
Form:
Liquid
Purification:
Immobilized Metal Affinity Chromatography
Isotype:
Mouse Fab fragment, His-tagged, kappa
Recommend Usage:
ImmunofluorescenceImmunohistochemistry (Frozen sections) (5 ug/mL)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (5 ug/mL)Immunoprecipitation (10 ug/mL)Western Blot (1 ug/mL)The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS, pH 7.2 (0.02% Proclin 300).
Storage Instruction:
Store at 4°C for up to 3 months. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt"s lymphomas, suggesting its importance in the normal function of this gene. [provided by RefSeq
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