High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.NEB extensively performs quality controls on all standard and high-fidelity(HF) restriction enzymes. Examples of nuclease contamination studies for someof our HF restriction enzymes are shown below.
EcoRI, NotI, and BamHI from multiple suppliers were tested in reactionscontaining a fluorescent labeled single stranded, double stranded blunt,3’overhang or 5’ overhang containing oligonucleotides. The percent degradationis determined by capillary electrophoresis and peak analysis. The resolution isat the single nucleotide level.
Product Source
AnE. colistrain that carries the cloned and modified SalI gene fromStreptomyces albusG (ATCC 49789)
This product is related to the following categories:
Methylation Sensitive Restriction Enzymes for Epigenetics Products,
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