BmtI has a High Fidelity version BmtI-HF™ (NEB #R3658).
High Fidelity (HF ™) Restriction Enzymes have 100% activity in CutSmart ™ Buffer; single-buffer simplicity means more straightforward and streamlined sample processing.HF enzymes also exhibit dramatically reduced star activity.HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 minutes with the flexibility to digest overnight without degradation to DNA.Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.
Product Source
An E.coli strain that carries the cloned BmtI gene from Bacillus megaterium S2 (S.K. Degtyarev).
Reagents Supplied
The following reagents are supplied with this product:
Store at (°C)
Concentration
NEBuffer™ 3.1
-20
10X
Notes:
BmtI is an neoschizomer of NheI.Star activity may result from extended digestion.
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