An E.coli strain that carries the CspCI gene from Citrobacter species 2144 (C. Nkenfou).
Reagents Supplied
The following reagents are supplied with this product:
Store at (°C)
Concentration
CutSmart® Buffer
-20
10X
S-adenosylmethionine (SAM)
-20
32 mM
Notes:
CspCI cleaves on both sides of its recognition sequence excising a ~35 base-pair fragment with 2-base 3´-overhangs.The exact positions of cleavage can vary ± one base depending on the sequence of the DNA flanking the recognition site and the digestion conditionsS-adenosylmethionine or SAM is supplied as a 32 mM solution in 0.005 M sulfuric acid and 10% ethanol. Under these conditions SAM is stable for up to 9 months when stored at -20°C.
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4000-520-616
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