Description:
PvuIIhasaHighFidelityversionPvuII-HF®(NEB#R3151).HighFidelity(HF®)RestrictionEnzymeshave100%activityinCutSmart™Buffer;single-buffersimplicitymeansmorestraightforwardandstreamlinedsampleprocessing. HFenzymesalsoexhibitdramaticallyreducedstaractivity. HFenzymesareallTime-Saver™ qualifiedandcanthereforecutsubstrateDNAin5-15minuteswiththeflexibilitytodigestovernightwithoutdegradationtoDNA. Engineeredwithperformanceinmind,HFrestrictionenzymesarefullyactiveunderabroaderrangeofconditions,minimizingoff-targetproducts,whileofferingflexibilityinexperimentaldesign.
PvuIIcrystals(IraSchildkrautandJosephBonventre,NewEnglandBiolabs)

ProductSource
AnE.colistrainthatcarriesthePvuIIgenefromProteusvulgaris(ATCC13315).ReagentsSupplied
Thefollowingreagentsaresuppliedwiththisproduct:
| Storeat(°C) | Concentration |
NEBuffer™3.1 | -20 | 10X |
Notes:
MayexhibitstaractivityinCutSmartBuffer.PvuIIdoesnotcutCAGm4CTG,whilecuttingatCAGm5CTGoccursatasignificantlyreducedrate.Forenzymesthatcannotbeheat-inactivated,werecommendusingacolumnforcleanup(suchastheMonarch®PCR&DNACleanupKit),orrunningthereactiononanagarosegelandthenextractingtheDNA(werecommendMonarchGelExtractionKit),orperformingaphenol/chloroformextraction.