Small RNAs include a variety of non-coding RNAs, such as miRNA, siRNA, snoRNA and snRNA. Recently such a small RNA is intensively studied, because the small RNA has been found to control many biological events. The Small RNA Marker consists of five single-stranded RNAs (ssRNA). The 20, 30, 40 and 50 bases RNAs are synthesized by chemically (non phosphorylated). The 100 bases RNA is synthesized by in vitro transcription. The Small RNA Marker is suitable for determinating size of small-size ssRNA in denaturing polyacrylamide gel electrophoresis. The Small RNA Marker can be visualized by ethidium bromide staining.
Quality Control Testing:
After 18 hrs incubation of the Small RNA Marker at 37°C, no visible degradation of the marker is observed in 12.5 % polyacrylamide / 7.5 M urea gel electrophoresis.
Recommend Usage:
1 uL/lane
Storage Buffer:
10 mM Tris-HCl (pH 8.0) buffer containing 1 mM EDTA (Ammonium acetate is slightly contained)
Storage Instruction:
Store at -80 °C. Repeated freeze/thaw cycles should be avoided.
Note:
The Small RNA Marker is not prepared for estimating of RNA amount. RNA is very sensitive to degradation by nucleases. To avoid damaging the Small RNA Marker , use extreme care during manipulations to prevent nuclease contamination. Wear gloves and use clean apparatus. Glassware should be pretreated with diethyl pyrocarbonate (DEPC). Nuclease-free disposable plasticware should be used. Solutions and reagents to mix the marker should be high grade and nuclease-free. To use, thaw the Small RNA Marker on ice and keep it on ice while using.
Protocol:
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Datasheet:
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Publication Reference
1.
TEX15 is an essential executor of MIWI2-directed transposon DNA methylation and silencing.Theresa Schöpp, Ansgar Zoch, Rebecca V Berrens, Tania Auchynnikava, Yuka Kabayama, Lina Vasiliauskaitė, Juri Rappsilber, Robin C Allshire, Dónal OCarroll.Nat Commun. 2020 Jul 27;11(1):3739. doi: 10.1038/s41467-020-17372-5.
2.
SPOCD1 is an essential executor of piRNA-directed de novo DNA methylation.Ansgar Zoch, Tania Auchynnikava, Rebecca V Berrens, Yuka Kabayama, Theresa Schöpp, Madeleine Heep, Lina Vasiliauskaitė, Yuvia A Pérez-Rico, Atlanta G Cook, Alena Shkumatava, Juri Rappsilber, Robin C Allshire, Dónal OCarroll.Nature. 2020 Aug;584(7822):635-639. doi: 10.1038/s41586-020-2557-5. Epub 2020 Jul 16.
3.
Disome and Trisome Profiling Reveal Genome-wide Targets of Ribosome Quality Control.Sezen Meydan, Nicholas R Guydosh.Mol Cell. 2020 Aug 20;79(4):588-602.e6. doi: 10.1016/j.molcel.2020.06.010. Epub 2020 Jul 1.
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