The RNA Marker Low Easy is supplied in a ready-to-use mixture of loading dye (containing formamide, EDTA sodium salt, bromphenol blue) and RNAs. It is prepared for denaturing polyacrylamide gel electrophoresis but not agarose gel elctrophoresis. The RNA Marker Low Easy has seven single-stranded RNAs, 20, 50, 100, 200, 300, 400 and 500 bases. The 20-base and 50-base RNA are synthesized by chemically (not phosphorylated), others are synthesized by in vitro transcription. In 5 ul of the RNA Marker Low Easy, each RNA amount is approximately 100 ng. It is useful for estimating RNA amount approximately. The RNA Marker Low Easy can be visualized by UV light after ethidium bromide staining.
Quality Control Testing:
After 18 hr incubation of the RNA Marker Low Easy at 37°C, no visible degradation of the marker is observed in 5 % polyacrylamide / 8M urea gel electrophoresis.
Recommend Usage:
5 uL is recommended for loading to a well (0.1 ug of each RNA / 5 uL)
Supplied Product:
RNA Loading buffer PARNA Loading buffer PA is manufactured for denaturing polyacrylamide gel electrophoresis but not agarose gel elctrophoresis. The loading buffer has a composition of 80% formamide, 10 mM EDTA sodium salt (pH 8.0), 0.025% bromphenol blue. Store RNA Loading buffer PA at -80 °C. Repeated freeze/thaw cycles should be avoided. It is 1 × to 2 × solution. Use more than one volume of RNA solution.
Storage Instruction:
Store at -80 °C. Repeated freeze/thaw cycles should be avoided.
Note:
RNA is very sensitive to degradation by nucleases. To avoid damaging the RNA Marker Low Easy, use extreme care during manipulations to prevent nuclease contamination. Wear gloves and use clean apparatus. Glassware should be pretreated with diethyl pyrocarbonate (DEPC). Nuclease-free disposable plasticware should be used. Solutions and reagents to mix the product should be high grade and nuclease-free. To use, thaw the RNA Marker Low Easy on ice and keep it on ice while using. For heat denaturation, transfer aliquot of the RNA Marker Low Easy to another tube, then heat it . Avoid repeated heat denaturizing.Formamide is suspected to be harmful. It is irritate to the eyes and skin. Wear appropriate gloves and safety glasses. Put a lid tightly at the time of storage.
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