GoldBio"s Reverse Transcriptase (RT) is a recombinant Moloney Murine Leukemia Virus (MMLV) reverse transcriptase with reduced RNase H activity. It is highly efficient at producing full-length cDNA from long RNA templates.
Our RT is an RNA-directed DNA polymerase which lacks 3´ → 5´ exonuclease activity and is capable of producing cDNA from as little as 50 pg of total RNA for real-time RT-PCR analysis and other applications.
Product Includes
- Reverse Transcriptase
- 10x RT Reaction Buffer
1X MMLV Reverse Transcriptase Reaction Buffer
- 50mM Tris-HCl, 75mM KCl, 3mM MgCl2, 10mM DTT, pH 8.3 at 25°C
Storage Buffer
- 50mM Tris-HCl, 50mM KCl, 1mM DTT, 0.1mM EDTA, 50% Glycerol, pH 7.5 at 25ºC
Source: The gene encoding recombinant MMLV Reverse Transcriptase with mutated RNase H domain is expressed in E. coli.
Unit Definition: One unit is defined as the amount of enzyme required to incorporate 1 nmol of dTTP into acid insoluble material in 10 minutes at 37°C using poly r(A)/oligo (dT) as a substrate.
Heat Inactivation: 65°C for 20 minutes.
Storage/Handling: Store at -20°C. Product may be shipped on blue ice.
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