TheCD154DetectionCocktailisdesignedfortheeasyidentificationandenumerationofactivatedantigen-specificCD4
+Tcellsbyflowcytometry.Tothisend,cellsarestimulatedwiththedesiredantigen
invitrotoinducetheexpressionofCD154andsimultaneouslyincubatedwiththeCD154DetectionCocktail.Besidesa
Fluorochrome-conjugatedCD154antibody,theCD154DetectionCocktailcontainsaCD40blockingantibodytopreventthedown-regulationofCD154surfaceexpressionandaCD28antibodytoprovideacostimulatorysignal.