Description
Qdot®800streptavidinconjugatecomprisesabiotin-bindingprotein(streptavidin)covalentlyattachedtoafluorescentlabel(Qdot®nanocrystal).Streptavidinhasaveryhighbindingaffinityforbiotin,andaconjugateofstreptavidiniscommonlyusedtogetherwithaconjugateofbiotinforspecificdetectionofavarietyofproteins,proteinmotifs,nucleicacids,andothermolecules(forexample,abiotinylatedprimaryantibodyboundtoaproteintargetcanbedetectedwithafluorescentlylabeledstreptavidin).Strategiessimilartothisareusedinmanydetectionprotocolsincludingwesternblots,flowcytometry,imagingandmicroscopy,andmicroplateassays,andarealsousedinpurificationworkflowstoachievetargetedfractionation.Qdot®nanocrystalconjugatesareprovidedas1µMsolutions.
ImportantFeaturesofQdot®StreptavidinConjugates:•
StreptavidinQdot®800conjugatehasemissionmaximaof~800nm•
Approximately5to10streptavidinsperQdot®nanocrystal•
Extremelyphotostableandbrightfluorescence•
Efficientlyexcitedwithsingle-lineexcitationsources•
Narrowemission,largeStokesshift•
Availableinmultiplecolors•
Idealforwesternblots,flowcytometry,imagingandmicroscopy,microplateassaysandmorePropertiesofQdot®NanocrystalsTheQdot®streptavidinconjugateisthesizeofalargemacromoleculeorprotein(~15–20nm)andrepresentsthebrightestclassofstreptavidindetectionreagents.Qdot®streptavidinconjugatesaremadefromananometer-scalecrystalofasemiconductormaterial(CdSe),whichiscoatedwithanadditionalsemiconductorshell(ZnS)toimprovetheopticalpropertiesofthematerial.TheQdot®705andQdot®800streptavidinconjugates,whichincludeCdSeTe,aremadeinasimilarfashion.Thiscore-shellmaterialisfurthercoatedwithapolymershellthatallowsthematerialstobeconjugatedto
BIOLOGicalmoleculesandtoretaintheiropticalproperties.
OtherFluorescentConjugatesofStreptavidinareAvailableWeofferseveralotherQdot®colorsortrytheQdot®StreptavidinSamplerKit,containingQdot®streptavidinconjugatesinsixcolors525,565,585,605,655,and705.Inadditiontonanocrystalconjugates,weofferawiderangeofstreptavidinsconjugatedtoAlexaFluor®dyes,OregonGreen®dye,enzymeconjugates,andtr
ADItionalfluorophoreslikeTexasRed®dye,fluorescein(FITC)andmore.
FindBiotinylatedConjugatesWeofferanextensivelineofbiotinylatedconjugatesforuseinbiotin-streptavidindetectionstrategies.
•Usetheprimaryantibodysearchtooltofindbiotinylatedprimaryantibodies
•Usethesecondaryantibodyselectortooltofindbiotinylatedsecondaryantibodiesandbiotinylatedanti-dyeandanti-haptenantibodies
BlockingEndogenousBiotinNaturallyoccurringbiotinscaninterferewithbiotin-streptavidindetectionschemes.Forexperimentsinvolvingfixedandperme
ABIlizedcells,tryourEndogenousBiotin-BlockingKittominimizethisinterference.
ForResearchUseOnly.Notintendedforanyanimalorhumantherapeuticordiagnosticuse.
RelatedLinks:LearnmoreaboutAvidin-BiotinDetection
TolearnmoreaboutQdot®Nanocrystals
ForResearchUseOnly.Notforuseindiagnosticprocedures.
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