Chickenswereimmunizedwithtwosyntheticpeptide/keyholelimpethemocyanin(KLH)conjugates.ThesesyntheticpeptidescorrespondedtodifferentregionsofthePogeneproduct,butaresharedbetweenthehuman(NP_000521,NCBI)andmouse(NP_032649,NCBI)sequences.Afterrepeatedinjections,immuneeggswerecollected,andtheIgYfractionswerepurifiedfromtheyolks.TheseIgYfractionswerethenaffinity-purifiedusingapeptidecolumn,andtheconcentrationsoftheeluatesadjustedto200ug/ml.Finally,equalvolumesofbothoftheseaffinitypurifiedanti-peptideantibodiesweremixed,andthepreparationwasfilter-sterilized.