TheCD4+RecentThymicEmigrantEnumerationKitisspecificallydesignedfortheanalysisofCD4+CD31+CD62L+CD45RO–Tcells(recentthymicemigrants,RTEs),enablinganeasyandreliableapproachtothequantificationofhumanthymicnaiveTcells.
ThekitcontainsallnecessaryreagentsfortheidentificationandenumerationofCD4+RTEsbyflowcytometry.
RTEsareasubpopulationofnaiveTcellscharacterizedbytheircontentofTcellreceptorexcisioncircles(TRECs)—stableDNAepisomesformedduringTcellreceptorrearrangement.TRECsarenotreplicatedduringmitosisanddiluteoutwitheachcellulardivision.Thedeterminationofacell’sTRECcontenthasbeenusedforthequantitationofthymicTcellproduction.TheoutputofRTEsfromthethymusandthehomeostaticproliferationofTcellscomprisethetotalnaiveTcellpool.
TheexpressionofCD31(plateletendothelialcelladhesionmolecule-1,PECAM-1)onCD4
+RTEsenablesenumerationofCD4
+RTEsbyflowcytometry,providinganalternativetothetime-consumingPCR-basedcharacterizationofCD4
+RTETRECcontent.TheidentificationofCD4
+RTEsisposs
IBLethroughanalysisfortheirexpressionofCD31,CD45RA,andCD62L,andalackofCD45ROexpression.CD4
+TcellslackingCD31expressionharborlittleornoTRECs,suggestingthattheyalreadyproliferatedintheperiphery.
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