Rabbit polyclonal antibody raised against partial recombinant of SARS-CoV-2 ORF3a.
Immunogen:
Recombinant protein corresponding to amino acids 126-275 of SARS-CoV-2 ORF3a.
Host:
Rabbit
Reactivity:
SARS-CoV-2
Specificity:
The antibody detects endogenous levels of SARS-CoV-2 ORF3a.
Form:
Liquid
Purification:
Affinity purification
Purity:
> 95% by SDS-PAGE
Isotype:
IgG
Recommend Usage:
ELISAWestern Blot (1:500)The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS, pH 7.2 (0.02% sodium azide).
Storage Instruction:
Store at 4°C for short term. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Datasheet:
Download
Applications
Western Blot
Western blot analysis of His-tag polyclonal antibody (1:500) and Goat Anti-Rabbit IgG (H&L) secondary antibody (HRP) (1:20000), Lane 1: 293F cell, Lane 2: 293F cell transfection pcDNA3.0-ORF3a-His. Western blot analysis of SARS-CoV-2 ORF3a polyclonal antibody and Goat Anti-Rabbit IgG (H&L) secondary antibody (HRP) (1:20000), Lane 3: 293F cell lysate (negative control), Lane 4: 293F cell transfection pcDNA3.0-ORF3a-His.