Rabbit polyclonal antibody raised against partial synthetic peptide of SARS-CoV-2 N.
Immunogen:
A synthetic peptide corresponding to amino acids 311-360 of SARS-CoV-2 N.
Host:
Rabbit
Reactivity:
SARS-CoV-2
Specificity:
The antibody detects endogenous levels of SARS-CoV-2 N.
Form:
Liquid
Purification:
Affinity purification
Purity:
> 95% by SDS-PAGE
Isotype:
IgG
Recommend Usage:
ELISAWestern Blot (1:500)The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS, pH 7.2 (0.02% sodium azide).
Storage Instruction:
Store at 4°C for short term. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Datasheet:
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Applications
Western Blot
Western blot analysis of Lane 1: N (SARS-CoV-2) Recombinant Protein (Cat # P6660), Lane 2: S1 (SARS-CoV-2) RBD Recombinant Protein (Cat # P6661), Lane 3: S (SARS-CoV-2) ECD Recombinant Protein (Cat # P6662) with SARS-CoV-2 N polyclonal antibody (1:500) and Goat Anti-Rabbit IgG (H&L) secondary antibody (HRP) (1:20000).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis was coated by N (SARS-CoV-2) Recombinant Protein (Cat # P6660), 100 uL/well at 2 ug/mL, was performed by loading SARS-CoV-2 N polyclonal antibody at various concentrations and Goat Anti-Rabbit IgG (H&L) secondary antibody (HRP) (1:10000).
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