Rabbit polyclonal antibody raised against synthetic peptide of human AKT1 (phospho S129).
Immunogen:
A synthetic peptide corresponding to amino acids 70-150 of human AKT1 (phospho S129).
Host:
Rabbit
Reactivity:
Human, Mouse, Rat
Specificity:
This antibody detects endogenous levels of Akt1 protein only when phosphorylated at S129.
Form:
Liquid
Purification:
Affinity purification
Isotype:
IgG
Recommend Usage:
ELISA (1:5000)Western Blot (1:500-2000)The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS (50% glycerol, 0.5% BSA and 0.02% sodium azide).
Storage Instruction:
Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Datasheet:
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Applications
Western Blot (Cell lysate)
Western Blot (Cell lysate) analysis of HepG2 cell lysate.
Enzyme-linked Immunoabsorbent Assay
Application Image
Western Blot (Cell lysate)
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Enzyme-linked Immunoabsorbent Assay
Gene Information
Entrez GeneID:
207
Protein Accession#:
P31749
Gene Name:
AKT1
Gene Alias:
AKT,MGC99656,PKB,PKB-ALPHA,PRKBA,RAC,RAC-ALPHA
Gene Description:
v-akt murine thymoma viral oncogene homolog 1
Omim ID:
164730, 181500
Gene Ontology:
Hyperlink
Gene Summary:
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq
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