Rabbit polyclonal antibody raised against synthetic peptide of ACPP.
Immunogen:
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human ACPP.
Host:
Rabbit
Reactivity:
Human
Form:
Liquid
Purification:
Ammonium sulfate precipitation
Recommend Usage:
Western Blot (1:1000)Immunohistochemistry (1:10-50)The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS (0.09% sodium azide)
Storage Instruction:
Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Datasheet:
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Publication Reference
1.
Prostatic acid phosphatase is not a prostate specific target.Quintero IB, Araujo CL, Pulkka AE, Wirkkala RS, Herrala AM, Eskelinen EL, Jokitalo E, Hellstrom PA, Tuominen HJ, Hirvikoski PP, Vihko PT.Cancer Res. 2007 Jul 15;67(14):6549-54.
2.
Identification of HLA-DRB1*1501-restricted T-cell epitopes from human prostatic acid phosphatase.Klyushnenkova EN, Kouiavskaia DV, Kodak JA, Vandenbark AA, Alexander RB.Prostate. 2007 Jul 1;67(10):1019-28.
3.
The Rab27a-binding protein, JFC1, regulates androgen-dependent secretion of prostate-specific antigen and prostatic-specific acid phosphatase.Johnson JL, Ellis BA, Noack D, Seabra MC, Catz SD.Biochem J. 2005 Nov 1;391(Pt 3):699-710.
Applications
Western Blot (Tissue lysate)
Western blot analysis of ACPP polyclonal antibody (Cat # PAB2990) in mouse lung tissue lysates (35 ug/lane). ACPP (arrow) was detected using the purified polyclonal antibody.
Formalin-fixed and paraffin-embedded human lung carcinomareacted with ACPP polyclonal antibody (Cat # PAB2990), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
This gene encodes an enzyme that catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is synthesized under androgen regulation and is secreted by the epithelial cells of the prostate gland. An alternatively spliced transcript variant encoding a longer isoform has been found for this gene. This isoform contains a transmembrane domain and is localized in the plasma membrane-endosomal-lysosomal pathway. [provided by RefSeq
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