Chicken polyclonal antibody raised against purified Rabbit Actin.
Immunogen:
Purified rabbit muscle actin.
Host:
Chicken
Reactivity:
Human, Mouse, Rabbit, Rat
Form:
Liquid
Purification:
Antigen affinity purification
Isotype:
IgY
Quality Control Testing:
This antibody was validated by western blot with nitrocellulose membranes (at a concentration of 1 ug/mL) using HRP-labeled goat anti-chicken IgY (1:5000 dilution) as the secondary reagent.Do NOT use this product with PVDF, or other nylon-based membranes, because this often produces unacceptably high backgrounds in chemiluminescence studies.
Recommend Usage:
ImmunocytochemistryWestern Blot(1:2500-1:5000)The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS, pH 7.2 (50% glycerol, 0.02% sodium azide)
Storage Instruction:
Store at -20°C and avoid from light.Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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