Rabbit polyclonal antibody raised against native ADA (Bovine).
Immunogen:
Native purified bovine ADA.
Host:
Rabbit
Reactivity:
Bovine
Form:
Lyophilized
Isotype:
IgG
Quality Control Testing:
Antibody Reactive Against Native Purified Protein.
Recommend Usage:
ELISA (1:5000 - 1:20000)Immunoprecipitation (1:100)Western Blot (1:500-1:5000)The optimal working dilution should be determined by the end user.
Storage Buffer:
Lyophilized from 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction:
Store at 4°C on dry atmosphere.After reconstitution with 0.1 mL of deionized water, store at -20°C or lower.Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Datasheet:
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Applications
Western Blot (Cell lysate)
Western Blot analysis of mouse pancreas, liver and Jurkat and Raji cells by ADA polyclonal antibody (Cat # PAB27925) at 1:5000. Lane 1: Reduced conditions(30 ul), Lane 2: purified ADA, Lane 3: Endogenous ADA in whole cell lysate from Jurkat and Raji cells (1:1 mixture) and Lane 4: Endogenous ADA in whole cell lysate from Mouse Pancreas and Liver (1:1 mixture).
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