Rabbit polyclonal antibody raised against native CAT (Bovine).
Immunogen:
Native purified bovine CAT .
Host:
Rabbit
Reactivity:
Bovine, Human, Mouse, Rat, Sheep
Form:
Liquid
Isotype:
IgG
Quality Control Testing:
Antibody Reactive Against Native Purified Protein.
Recommend Usage:
ELISA (1:20000)Western Blot (1:10000-1:50000)The optimal working dilution should be determined by the end user.
Storage Buffer:
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction:
Store at 4°C. For long term storage store at -20°C.Aliquot after reconstitution to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Datasheet:
Download
Applications
Western Blot (Recombinant protein)
Western Blot analysis by CAT polyclonal antibody (Cat # PAB27918) at 1:1000. Lane 1: Reduced at about 1ug (4% beta-Mercaptoethanol, boiled for 5 minutes). Lane 2: Non-reduced conditions at about 1 ug. Detected CAT at 59.9 kDa and 55kDa.