Rabbit polyclonal antibody raised against synthetic phosphopeptide of ADD1.
Immunogen:
Synthetic phosphopeptide corresponding to residues surrounding S726 of human ADD1.
Sequence:
T-P-Sp-F-L
Host:
Rabbit
Reactivity:
Human, Mouse, Rat
Form:
Liquid
Purification:
Affinity purification
Recommend Usage:
Western Blot (1:500-1:1000)Immunohistochemistry (1:50-100)The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction:
Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Datasheet:
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Applications
Western Blot (Cell lysate)
Western blot analysis of extract from HT-29 cells untreated or treated with Doxorubicin (1 mM, 30 min), using ADD1 (phospho S726) polyclonal antibody (Cat # PAB25323).
Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described. [provided by RefSeq
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