Rabbit polyclonal antibody raised against synthetic peptide of CD36.
Immunogen:
A synthetic peptide corresponding to amino acids 100-200 of human CD36.
Host:
Rabbit
Reactivity:
Bovine, Human, Mouse, Primates, Rat
Form:
Liquid
Recommend Usage:
Immunocytochemistry (1:50-1:200)Immunofluorescence (1:50-1:200)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:400)Western Blot (1:500-1:2000)The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS buffer (0.02% sodium azide)
Storage Instruction:
Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Datasheet:
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Publication Reference
1.
Lipid oversupply induces CD36 sarcolemmal translocation via dual modulation of PKCζ and TBC1D1: an early event prior to insulin resistance.Zhu B, Li MY, Lin Q, Liang Z, Xin Q, Wang M, He Z, Wang X, Wu X, Chen GG, Tong PC, Zhang W, Liu LZ.Theranostics. 2020 Jan 1;10(3):1332-1354. doi: 10.7150/thno.40021. eCollection 2020.
2.
Lipolysis in Brown Adipocytes Is Not Essential for Cold-Induced Thermogenesis in Mice.Shin H, Ma Y, Chanturiya T, Cao Q, Wang Y, Kadegowda AKG, Jackson R, Rumore D, Xue B, Shi H, Gavrilova O, Yu L.Cell Metab. 2017 Nov 7;26(5):764-777.e5. doi: 10.1016/j.cmet.2017.09.002. Epub 2017 Oct 5.
Applications
Western Blot (Cell lysate)
Detection of CD36 in human adipocyte extract (30 ug) using CD36 polyclonal antibody (Cat # PAB12463). Lane 1 : 0.5 ug/mL ; lane 2 : 2 ug/mL .
The protein encoded by this gene is the fourth major glycoprotein of the platelet surface and serves as a receptor for thrombospondin in platelets and various cell lines. Since thrombospondins are widely distributed proteins involved in a variety of adhesive processes, this protein may have important functions as a cell adhesion molecule. It binds to collagen, thrombospondin, anionic phospholipids and oxidized LDL. It directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes and it binds long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport. Mutations in this gene cause platelet glycoprotein deficiency. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
Other Designations:
CD36 antigen,CD36 antigen (collagen type I receptor, thrombospondin receptor),PAS-4 protein,cluster determinant 36,fatty acid translocase,glycoprotein IIIb,leukocyte differentiation antigen CD36,platelet glycoprotein IV,scavenger receptor class B, member
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