Rabbit polyclonal antibody raised against synthetic phosphopeptide of ABL1/ABL2.
Immunogen:
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y393/Y429 of human ABL1/ABL2.
Sequence:
DTYpTA
Host:
Rabbit
Theoretical MW (kDa):
210
Reactivity:
Human, Mouse
Specificity:
ABL1/2 (phospho-Tyr393/429) Antibody detects endogenous levels of ABL1/2 only when phosphorylated at tyrosine393/429.
Form:
Liquid
Purification:
Immunoaffinity purification
Concentration:
1 mg/mL
Quality Control Testing:
Antibody Reactive Against Synthetic Peptide.
Recommend Usage:
Immunohistochemistry (1:50-1:100)Immunofluorescence (1:100-1:200)The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction:
Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Datasheet:
Download
Publication Reference
1.
Autoinhibition of c-Abl.Pluk H, Dorey K, Superti-Furga G.Cell. 2002 Jan 25;108(2):247-59.
2.
c-Abl has high intrinsic tyrosine kinase activity that is stimulated by mutation of the Src homology 3 domain and by autophosphorylation at two distinct regulatory tyrosines.Brasher BB, Van Etten RA.J Biol Chem. 2000 Nov 10;275(45):35631-7.
3.
JAK-STAT signaling activated by Abl oncogenes.Danial NN, Rothman P.Oncogene. 2000 May 15;19(21):2523-31.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ABL1/ABL2 (phospho Y393/429) polyclonal antibody (Cat # PAB12190).
Immunofluorescence
Immunofluorescence staining of methanol-fixed HeLa cells using ABL1/ABL2 (phospho Y393/429) polyclonal antibody (Cat # PAB12190, red).
The ABL1 protooncogene encodes a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation, cell division, cell adhesion, and stress response.Activity of c-Abl protein is negatively regulated by its SH3 domain, and deletion of the SH3 domain turns ABL1 into an oncogene.The t(9;22) translocation results in the head-to-tail fusion of the BCR (MIM:151410) and ABL1 genes present in many cases of chronic myelogeneous leukemia.The DNA-binding activity of the ubiquitously expressed ABL1 tyrosine kinase is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function for ABL1.The ABL1 gene is expressed as either a 6- or 7-kb mRNA transcript, with alternatively spliced first exons spliced to the common exons 2-11. [provided by RefSeq
This gene encodes a member of the Abelson family of nonreceptor tyrosine protein kinase. The protein is highly similar to the ABL1 protein, including the tyrosine kinase, SH2 and SH3 domains, and has a role in cytoskeletal rearrangements by its C-terminal F-actin- and microtubule-binding sequences. This gene is expressed in both normal and tumor cells, and is involved in translocation with the ETV6 gene in leukemia. Multiple alternatively spliced transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq
购物车为空，快去购物吧！
4000-520-616
咨询顾问
特别 提示
Download
