Rabbit polyclonal antibody raised against full length recombinant SARS orf1ab.
Immunogen:
A synthetic peptide (conjugated with BSA) corresponding to amino acids 204-221 of SARS orf1ab.
Host:
Rabbit
Reactivity:
Viruses
Specificity:
This antibody is directed against SARS-Coronavirus membrane protein.
Form:
Liquid
Quality Control Testing:
Antibody Reactive Against Synthetic Peptide.
Recommend Usage:
Western Blot (1:1000)Immunofluorescence (1:1000)Immunoprecipitation (1:60)Immunoelectron Microscopy (1:200)The optimal working dilution should be determined by the end user.
Storage Buffer:
In antiserum (0.01% sodium azide)
Storage Instruction:
Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Datasheet:
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Publication Reference
1.
Ultrastructure and origin of membrane vesicles associated with the severe acute respiratory syndrome coronavirus replication complex.Snijder EJ, van der Meer Y, Zevenhoven-Dobbe J, Onderwater JJ, van der Meulen J, Koerten HK, Mommaas AM.J Virol. 2006 Jun;80(12):5927-40.
2.
Unique and conserved features of genome and proteome of SARS-coronavirus, an early split-off from the coronavirus group 2 lineage.Snijder EJ, Bredenbeek PJ, Dobbe JC, Thiel V, Ziebuhr J, Poon LL, Guan Y, Rozanov M, Spaan WJ, Gorbalenya AE.J Mol Biol. 2003 Aug 29;331(5):991-1004.
Applications
Immunofluorescence
Immunofluorescence Microscopy using orf1ab polyclonal antibody (Cat # PAB11369), 6 hrs post infection Vero-E6 cells.Personal Communication, Eric Snijder, LeidenUniversity Medical Center, Leiden, Netherlands.
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