Rabbit polyclonal antibody raised against full length recombinant SARS Coronavirus 3CL Protease.
Immunogen:
Recombinant protein corresponding to full length SARS Coronavirus 3CL Protease.
Host:
Rabbit
Reactivity:
SARS-CoV
Specificity:
This protein A purified antibody is directed against SARS Coronavirus 3CL Protease.
Form:
Lyophilized
Purification:
The product was purified from monospecific antiserum by protein A affinity purification.
Isotype:
IgG
Recommend Usage:
ELISA (1:10000-1:50000)ImmunofluorescenceWestern Blot (1:2000-1:10000)The optimal working dilution should be determined by the end user.
Storage Buffer:
Lyophilized from 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide).
Storage Instruction:
Store at 4°C on dry atmosphere prior to restoration.After reconstitution with 0.1 mL of deionized water (or equivalent), for extended storage aliquot contents and freeze at -20°C or below.Aliquot to avoid repeated freezing and thawing.Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Datasheet:
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Publication Reference
1.
Expression and purification of SARS coronavirus proteins using SUMO-fusions.Zuo X, Mattern MR, Tan R, Li S, Hall J, Sterner DE, Shoo J, Tran H, Lim P, Sarafianos SG, Kazi L, Navas-Martin S, Weiss SR, Butt TR.Protein Expr Purif. 2005 Jul;42(1):100-10. Epub 2005 Feb 23.
2.
Enhanced expression and purification of membrane proteins by SUMO fusion in Escherichia coli.Zuo X, Li S, Hall J, Mattern MR, Tran H, Shoo J, Tan R, Weiss SR, Butt TR.J Struct Funct Genomics. 2005;6(2-3):103-11.
3.
SUMO fusions and SUMO-specific protease for efficient expression and purification of proteins.Malakhov MP, Mattern MR, Malakhova OA, Drinker M, Weeks SD, Butt TR.J Struct Funct Genomics. 2004;5(1-2):75-86.
Applications
Western Blot (Recombinant protein)
Western blot using SARS-CoV 3CL Protease polyclonal antibody (Cat # PAB11368) shows detection of a 34- KDa band corresponding to the protein.Approx. 100 ng of protein was loaded for SDS-PAGE and transferred onto nitrocellulose.The blot was incubated with a 1:5,000 dilution of the antibody at room temperature for 1 h.
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