Rabbit polyclonal antibody raised against recombinant ATG12.
Immunogen:
Recombinant protein corresponding to Saccharomyces cerevisiae ATG12.
Host:
Rabbit
Reactivity:
Yeast
Form:
Lyophilized
Quality Control Testing:
Antibody Reactive Against Recombinant Protein.
Recommend Usage:
ELISA (1:1000-1:5000)Western Blot (1:2000)The optimal working dilution should be determined by the end user.
Storage Buffer:
Lyophilized from 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction:
Store at 4°C. For long term storage store at -20°C.Aliquot after reconstitution to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Datasheet:
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Publication Reference
1.
Mouse Apg16L, a novel WD-repeat protein, targets to the autophagic isolation membrane with the Apg12-Apg5 conjugate.Mizushima N, Kuma A, Kobayashi Y, Yamamoto A, Matsubae M, Takao T, Natsume T, Ohsumi Y, Yoshimori T.J Cell Sci. 2003 May 1;116(Pt 9):1679-88.
2.
Formation of the approximately 350-kDa Apg12-Apg5.Apg16 multimeric complex, mediated by Apg16 oligomerization, is essential for autophagy in yeast.Kuma A, Mizushima N, Ishihara N, Ohsumi Y.J Biol Chem. 2002 May 24;277(21):18619-25. Epub 2002 Mar 15.
3.
The pre-autophagosomal structure organized by concerted functions of APG genes is essential for autophagosome formation.Suzuki K, Kirisako T, Kamada Y, Mizushima N, Noda T, Ohsumi Y.EMBO J. 2001 Nov 1;20(21):5971-81.
Applications
Western Blot (Recombinant protein)
Immunoblot of APG12 fusion protein.ATG12 polyclonal antibody (Cat # PAB11358) generated by immunization with recombinant yeast ATG12 was tested by immunoblot against yeast lysates expressing the ATG12-GFP fusion protein and other UBL fusion proteins.All UBLs possess limited homology to Ubiquitin and to each other, therefore it is important to know the degree of reactivity of each antibody against each UBL.Panel A shows total protein staining using ponceau.Panel B shows positions of free GFP or GFP containing recombinant proteins present in each lysate preparation after reaction with a 1:1,000 dilution of anti-GFP followed by reaction with a 1:15,000 dilution of HRP Donkey-a-Goat IgG MX.Panel C shows specific reaction with ATG12 using a 1:2,000 dilution of ATG12 polyclonal antibody (Cat # PAB11358) followed by reaction with a 1:15,000 dilution of HRP Goat-a-Rabbit IgG MX.All primary antibodies were diluted in TTBS buffer supplemented with 5% non-fat milk and incu
Enzyme-linked Immunoabsorbent Assay
Application Image
Western Blot (Recombinant protein)
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Enzyme-linked Immunoabsorbent Assay
Gene Information
Entrez GeneID:
852518
Gene Name:
ATG12
Gene Alias:
APG12
Gene Description:
Atg12p
Gene Ontology:
Hyperlink
Other Designations:
Ubiquitin-like modifier, conjugated via an isopeptide bond to a lysine residue of Atg5p by the E1 enzyme, Atg7p, and the E2 enzyme, Atg10p, a step that is essential for autophagy
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