Goat polyclonal antibody raised against native ALDOA.
Immunogen:
Native purified ALDOA from rabbit muscle.
Host:
Goat
Reactivity:
Rabbit
Form:
Lyophilized
Quality Control Testing:
Antibody Reactive Against Native Purified Protein.
Recommend Usage:
Sandwich ELISA (1:10000-1:40000)Immunoprecipitation (5 ul)The optimal working dilution should be determined by the end user.
Storage Buffer:
In 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction:
Store at 4°C on dry atmosphere.After reconstitution with deionized water, store at -20°C or lower.Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Datasheet:
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Applications
Western Blot (Cell lysate)
IgG purified ALDOA polyclonal antibody (Cat # PAB11351) to rabbit muscle aldolase was used at a 1:1000 dilution to detect human aldolase by Western blot.A whole cell lysate prepared from human derived A293 cells was loaded on a 4-12% tris glycine gradient gel for SDS-PAGE.The gel was transferred to nitrocellulose using standard techniques. Antibody reaction with the membrane occurred overnight at 4°C in TTBS supplemented with 2% non-fat dry milk.Color was allowed to develop using Super Signal West Pico Chemiluminescent Substrate (PIERCE).This antibody clearly detects a band at ~41 KDa consistent with human aldolase.
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4000-520-616
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