Chicken anti-mouse IgG recognizes mouse IgG whole molecule. This secondary antibody was purified using antigen affinity chromatography. The antibody is conjugated with Fluorescein.
Immunogen:
Mouse IgG whole molecule
Host:
Chicken
Reactivity:
Mouse
Form:
Lyophilized
Conjugation:
FITC
Purification:
This product was prepared from monospecific antiserum by immunoaffinity chromatography, followed by solid phase adsorption(s) to remove any unwanted reactivities.
Conjugation Note:
3.5 moles FITC per mole of chicken IgG
Recommend Usage:
Immunofluorescence (1:1000-1:5000)Flow Cytometry (1:500-1:2500)The optimal working dilution should be determined by the end user.
Storage Buffer:
Lyophilized from 20 mM potassium phosphate, 150 mM NaCl, pH 7.2 (0.01% (w/v) sodium azide, 10 mg/mL BSA (Immunoglobulin and Protease free))
Storage Instruction:
Store at 4°C on dry atmosphere.After reconstitution with 1 mL of deionized water, store at -20°C or lower.Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Datasheet:
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Publication Reference
1.
Conjugation of fluorescein isothiocyanate to antibodies. I. Experiments on the conditions of conjugation.The TH, Feltkamp TE.Immunology. 1970 Jun;18(6):865-73.
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