Rabbit polyclonal antibody raised against synthetic phosphopeptide of ABL1.
Immunogen:
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y134 of human ABL1.
Host:
Rabbit
Reactivity:
Human
Form:
Liquid
Purification:
Protein G purification
Recommend Usage:
Immunohistochemistry (1:50-100)Dot Blot (1:500)The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS (0.09% sodium azide)
Storage Instruction:
Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Datasheet:
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Publication Reference
1.
Chronic myeloid leukaemia: an investigation into the role of Bcr-Abl-induced abnormalities in glucose transport regulation.Barnes K, McIntosh E, Whetton AD, Daley GQ, Bentley J, Baldwin SA.Oncogene. 2005 May 5;24(20):3257-67.
2.
Interpretation of submicroscopic deletions of the BCR or ABL gene should not depend on extra signal-FISH: problems in interpretation of submicroscopic deletion of the BCR or ABL gene with extra signal-FISH.Kim YR, Cho HI, Yoon SS, Park S, Kim BK, Lee YK, Chun H, Kim HC, Lee DS.Genes Chromosomes Cancer. 2005 May;43(1):37-44.
3.
Identification of mcl-1 as a BCR/ABL-dependent target in chronic myeloid leukemia (CML): evidence for cooperative antileukemic effects of imatinib and mcl-1 antisense oligonucleotides.Aichberger KJ, Mayerhofer M, Krauth MT, Skvara H, Florian S, Sonneck K, Akgul C, Derdak S, Pickl WF, Wacheck V, Selzer E, Monia BP, Moriggl R, Valent P, Sillaber C.Blood. 2005 Apr 15;105(8):3303-11. Epub 2004 Dec 30.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with ABL1 (phospho Y134) polyclonal antibody (Cat # PAB0396) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
Dot Blot (Peptide)
Dot blot analysis of ABL1 (phospho Y134) polyclonal antibody (Cat # PAB0396) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed.
The ABL1 protooncogene encodes a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation, cell division, cell adhesion, and stress response.Activity of c-Abl protein is negatively regulated by its SH3 domain, and deletion of the SH3 domain turns ABL1 into an oncogene.The t(9;22) translocation results in the head-to-tail fusion of the BCR (MIM:151410) and ABL1 genes present in many cases of chronic myelogeneous leukemia.The DNA-binding activity of the ubiquitously expressed ABL1 tyrosine kinase is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function for ABL1.The ABL1 gene is expressed as either a 6- or 7-kb mRNA transcript, with alternatively spliced first exons spliced to the common exons 2-11. [provided by RefSeq
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